Dental pulp pluripotent-like stem cells (DPPSC), a new stem cell population with chromosomal stability and osteogenic capacity for biomaterials evaluation

Table 1 Primer pairs used to assess osteoblast differentiation in the RT-PCR and qRT-PCR amplification

Gene	Accession number	Forward primer (5′-3′)	Reverse primer (5′-3′)	Product size (bp)	Use
OCT 3/4	NM_002701	GTGGAGAGCAACTCCGAT G	TGCAGAGCTTTGATGTCCTG	122	RT-PCR qRT-PCR
NANOG	NM_024865	CAGAAGGCCTCAGCACCTAC	ATTGTTCCAGGTCTGGTTGC	111	RT-PCR qRT-PCR
ALP	NM_000478	GGACATGCAGTACGAGCTGA	GTCAATTCTGCCTCCTTCCA	133	RT-PCR
ALP	NM_000478	CCGTGGCAACTCTATCTTTGG	GCCATACAGGATGGCAGTGA	79	qRT-PCR
COL1	NM_000088	ACTGGTGAGACCTGCGTGTA	CAGTCTGCTGGTCCATGTA	263	RT-PCR
COL1	NM_000088	CCCTGGAAAGAATGGAGATGAT	ACTGAAACC TCTGTGTCCCTTCA	139	qRT-PCR
OC	NM_199173	GTGCAGCCTTTGTGTCCAA	GCTCACACACCTCCCTCCT	129	RT-PCR
OC	NM_199173	AAGAGACCCAGGCGCTACC T	AAC TCGTCACAGTCCGGATTG	110	qRT-PCR
RUNX2	NM_001146038	TTACTGTCATGGCGGGTAAC	GGTTCCCGAGGTCCATCTA	220	RT-PCR
RUNX2	NM_001146038	AGCAAGGTTCAACGATCTGAGAT	TTTGTGAAGACGGTTATGGTCAA	81	qRT-PCR
VLA4	NM_000885	CGAACCGATGGCTCCTAGTG	CACGTCTGGCCGGGATT	115	RT-PCR
ITGα3	NM_002204	TCCGAGTCAATGTCCACAGA	GCTGGGCTACCCTATTCCTC	88	RT-PCR qRT-PCR
ITGαV	NM_002210	CCTTGCTGCTCTTGGAAC TC	ATTCTGTGGCTGTCGGAGAT	74	RT-PCR qRT-PCR
GAPDH	NM_002046	CTGGTAAAGTGGATATTGTTGCCAT	TGGAATCATATTGGAACATGTAAACC	81	RT-PCR qRT-PCR

OCT-3/4 octamer-binding transcription factor 4, Nanog Nanog homeobox, ALP alkaline phosphatase, COL1 type I collagen, OC osteocalcin, RUNX2 Runt-related protein 2, VLA-4 Integrin alpha4beta1, ITGα3 integrin alpha 3, ITGαV integrin, alpha V, GAPDH glyceraldehyde-3-phosphate dehydrogenase

ISSN: 2661-8850