Fig. 2
From: Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis
The ACA mRNA is asymmetrically distributed at the posterior of the streaming cells. a Left: Cartoon depicting the distribution of cells within a stream. Each cell was characterized as either being at the end, in the middle or at the beginning of a stream based on its position relative to the aggregate center. Right: Eccentricity measurement of ACA-YFP/aca − and cAR1-YFP/car1/3 −/− cells at the various positions within streams (nACA = 45, ncAR1 = 24). b The simulated estimate of mRNA units across cells is plotted for ACA-YFP/aca − and cAR1-YFP/car1/3 −/− cells, as a function of their position in a stream. Boxes show the 50% confidence region from the median (dashed black line). The bars cover a region with 99% confidence level from the median. All data points beyond this confidence level are considered as outliers and shown with black dots. The statistical significance is inferred by the t-test, *** represents p < 0.001 (n = 15–52). c-d The average proportion of mRNA and its corresponding protein at the posterior of cells is presented for ACA-YFP/aca − and cAR1-YFP/car1/3 −/− cells (see Supplemental experimental procedure for details; n = 48–67)