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Fig. 3 | BMC Cell Biology

Fig. 3

From: Adenylyl cyclase mRNA localizes to the posterior of polarized DICTYOSTELIUM cells during chemotaxis

Fig. 3

ACA translation occurs in the cytoplasm followed by localization to the posterior of the polarized cells. a Representative phase contrast (upper panel) and confocal fluorescent (lower panel) images of ACA-YFP/aca − cells treated with 1.6 mM CHX for 2 h. Fluorescent recovery was monitored after CHX removal. ACA-YFP protein is depicted in green. The white star indicates the location of the aggregation center. Data are representative of at least 3 independent experiments. b Representative maximum intensity projections of confocal fluorescent images of ACA-YFP/aca − cells depicting DAPI (blue) and ACA mRNA (red). The white star indicates the location of the aggregation center. Data are representative of at least 3 independent experiments. c Representative phase contrast (upper panel) and confocal fluorescent (lower panel) images of cAR1-YFP/car1/3 −/− treated with 1.6 mM CHX for 2 h. Fluorescent recovery was monitored after CHX removal. cAR1-YFP protein is depicted in green. The white star indicates the location of the aggregation center. Data are representative of at least 3 independent experiments. d Representative maximum intensity projections of confocal fluorescent images depicting DAPI (blue) and cAR1 mRNA (red). The white star indicates the location of the aggregation center. Data are representative of at least 3 independent experiments

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