Fig. 1

Synthesis and characterisation of novel resveralogues. a Structures of resveralogues 1–6. Compounds are: 1 resveratrol, 2 resveratrol’s primary metabolite, dihydroresveratrol, 3 (E)-N-(4-(3,5-dimethoxystyryl) phenyl)methanesulfonamide, 4 (E)-N-(4-(3,5-dihydroxystyryl)phenyl)acetamide, 5 (E)-5-(4-(3,5-dimethoxystyryl)phenyl)-1H–tetrazole and 6 (E)-5-(2-(3,5-dimethoxystyryl)phenyl)-1H–tetrazole. b Scheme of synthesis of compounds 3–6 (see Methods for details). c Fluorescence determination of SIRT1 activity in vitro in the presence of 25 μM each compound, normalised against resveratrol (1) and vehicle only control (0). Data are presented as fold change (mean ± SD) in activity normalised to enzyme-only (0) and resveratrol (1), such that 0 represents no activation, and 1.0 indicates activation equivalent to that observed with resveratrol 1. The experiment was carried out in 3 replicates. The numbers on the X axis (1–6) refer to the identity of each resveralogue as indicated above. Uncertainty was calculated by subjecting the standard deviation of the control, Resveratrol and compound data to combination using standard methods for propagation of uncertainty [49]