Fig. 6

Effects of resveratrol treatment in cells grown under serum starvation conditions. a Cell numbers of NHDF fibroblasts following treatment with 5 μM resveratrol for 24 h under conditions of serum starvation. Experiments were carried out in triplicate for three biological replicates. (2 way ANOVA). b Proliferation index was assessed for NHDF fibroblasts following treatment with 5 μM resveratrol for 24 h under conditions of serum starvation as assessed by Ki67 immunofluorescence (> 400 nuclei counted per sample). c Senescence associated β-galactosidase following NHDF fibroblasts following treatment with 5 mM resveratrol for 24 h under conditions of serum starvation was determined by manually counting the percentage of SA-β gal positive cells in each treated or control population. n > 300 for each sample. Statistical significance is indicated by *** = p < 0.0005 (2 way ANOVA). d Changes to splicing factor mRNA levels in NHDF fibroblasts following treatment with 5 μM resveratrol for 24 h under conditions of serum starvation determined by qRTPCR. Control = vehicle only. Green indicates up-regulated genes, red denotes down-regulated genes. The colour scale refers to fold-change in expression. Data are derived from duplicate testing of 3 biological replicates