Fig. 5

Studies on requirement of functional ETC for radiation protection offered by mannan. The requirement of functionality ETC in radiation protection was assessed using inhibitors of complex I and Complex III. Briefly cells were treated with (a) Rotenone, inhibitor of complex I, (b) Antimycin A, inhibitor of complex III, (c) Both Rotenone and antimycin A, 30 min prior to mannan treatment and/or irradiation followed by SRB uptake after 96 h as described under materials and methods. Results are expressed as fold changes in cell proliferation ± SD of three independent experiments. Differences were designated significant at values *p < 0.05, ** p < 0.001 and *** p < 0.0001 and were labeled as # compared with the sham irradiated control group, * compared with the radiation group, and $ compared with radiation + mannan group