Fig. 7

Tan IIA activates mitochondrial fission via the JNK-Mff pathways. a-c Western blotting was conducted to verify the alteration of JNK phosphorylation and Mff expression. d-f Immunofluorescence assays for JNK phosphorylation and Mff expression. Tan IIA treatment significantly increased the expression of p-JNK and Mff. g-h Mitochondrial fission was observed via immunofluorescence using the mitochondrial-specific antibody Tom-20. The average length of the mitochondria was recorded. SP600125, an inhibitor of JNK, was added to the Tan IIA-treated cells to prevent JNK activation. *p < 0.05