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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Highly efficient correction of structural mutations of 450 kb KIT locus in kidney cells of Yorkshire pig by CRISPR/Cas9

Fig. 1

sgRNAs design and targeting efficiency measurement. a Schematic diagram of the target sites of sgRNAs designed for targeting introns 16 and 17 of the porcine KIT gene. Blue rectangles indicate exons and dark lines indicate introns. Half arrows indicate the sequence of the guide segment of sgRNAs. Red bases represent the NGG nucleotide protospacer adjacent motif (PAM) sequences. b The frequency of CRISPR/Cas9-induced mutations determined by the T7E1 assay. M, DNA marker; NC, negative control; US, unsorted; S, sorted. Red arrowheads indicate the expected positions of DNA bands cleaved by mismatch-sensitive T7E1. The numbers along the bottom of the gel indicate the mutation percentages calculated based on the band intensities using Image J software. c Sequence analysis of cloned PCR products. DNA sequences of the wild-type (WT) and mutant clones, with CRISPR/Cas9 recognition sites shown in red and PAM sequences in blue. Dashes and purple letters indicate deleted and inserted bases, respectively

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