Fig. 2

miR-3198 gain-of-function and loss-of-function experiments. Results of real-time RT-PCR analysis for miR-3198 (a, b), OPG and RANKL (c, d) expression in HPL cells after transfection of miR-3198 inhibitor (a, c) and miR-3198 mimic (b, d). n = 3. Biological triplicated. Fold change from the control is shown. Open bar indicates the fold change of OPG expression, and close bar indicates that of RANKL expression (c, d). The concentrations of OPG as measured by ELISA after transfection of miR-3198 inhibitor (e) and miR-3198 mimic (f) are shown (n = 3). Results of real-time PCR analysis for miR-1207 (g, h), OPG and RANKL (i, j) expression in HPL cells under the transfection of miR-1207 inhibitor (g, i) and miR-1207 mimic (h, j). n = 3. Biological triplicated. Fold changes from the control are shown. * indicates P < 0.05 versus control, and NS indicating there was no significant difference between samples