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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Downregulation of lncRNA CCAT1 enhances 5-fluorouracil sensitivity in human colon cancer cells

Fig. 5

Mechanism of CCAT1 on the sensitivity of cells to 5-FU. a. The relative expression of miRNAs in HCT 116 and HCT 116/5-FU cells was assessed using qRT-PCR. b. The relative expression miRNAs in HT-29 and HT-29/5-FU cells was determined by qRT-PCR. c. The relative expression of miRNAs in HCT 116/5-FU cells was determined using qRT-PCR after siRNA-CCAT1 transfection. d. The relative expression of miRNAs in HT-29/5-FU cells was examined by qRT-PCR after siRNA-CCAT1 transfection. e. The protein expressions of γ-H2AX, p53 and c-Myc in HCT 116 and HT-29 cell lines were determined by Western blot after pcDNA3.1-CCAT1 transfection. F. HCT 116/5-FU and HT-29/5-FU cells were transfected with siRNA-CCAT1, then the protein levels of γ-H2AX, p53 and c-Myc were evaluated by Western blot. *P < 0.05, **P < 0.05, compared with HCT 116, HT-29 or NC group. All data were means ± SD

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