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Fig. 2 | BMC Molecular and Cell Biology

Fig. 2

From: The role of Patronin in Drosophila mitosis

Fig. 2

In prometaphase and metaphase cells Patronin-enriched MT bundles are separated from the centrosomes. Cells expressing Patronin-GFP and Cherry-tubulin were fixed and stained with chicken anti-GFP, mouse anti-α-tubulin and rabbit anti-Spd2 antibodies, which were detected using secondary antibodies conjugated with Alexa Fluor 488 (green), Alexa Fluor 568 (red) and Alexa Fluor 660 (far red; white), respectively. Note that in prometaphase and metaphase cells the fluorescent MT bundles do not reach the centrosomes, whereas in prophase and telophase cells at least some bundles contact the centrosomes. Scale bar, 5 μm

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