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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Dlx5 and Dlx6 can antagonize cell division at the G1/S checkpoint

Fig. 3

Dlx proteins do not promote cell cycle exit. a Proliferating C2C12 cells were co-transfected with a nuclear GFP-encoding plasmid and either a Dlx5-encoding plasmid or an empty vector and seeded onto poly-D-Lysine-coated coverslips. Ki67 and GFP were detected at the times shown post-transfection. The proportion of actively cycling (Ki67-positive) cells was determined for both the transfected and non-transfected populations on each slide. Bars represent the averages from a minimum of three experiments ± SEM, with at least 300 GFP-positive cells counted in total. b As for A, with Dlx6-encoding plasmid. No significant difference was detected at any given time point for any two-way comparison (unpaired t-tests)

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