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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Isolation of fresh endothelial cells from porcine heart for cardiovascular studies: a new fast protocol suitable for genomic, transcriptomic and cell biology studies

Fig. 1

Timeline of endothelial cell isolation protocol. Isolation protocol takes around 1–2 h to complete depending on the skill level of a scientist. Isolation protocol includes: isolation of the heart, perfusion (setuping heart for perfusion, PBS perfusion and perfusion of Collagenase solution), Incubation (37 °C water bath), collection of endothelial cells, pooling and plating (includes time needed for sentrifucation, removal of supernatant, pooling cells and plating them on fibronectin gelating-coated plates). Cell culturing includes: attachment of endothelial cells, washing and culturing time. Cells can be used for experiments or passaged when plates are 80–90% confluent

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