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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

Fig. 4

SD (a) and polymerase (b) activity analysis of polymerase I large fragment from Psychrobacillus sp. (PB pol I LF) and its D422A variant (PB D422A). Activities have been measured using the time-resolved strand-displacement and polymerase activity assay, respectively, at 25 °C in 50 mM BIS-Tris propane pH 8.5, 100 mM NaCl, 5 mM MgCl2, 1 mM DTT, 0.2 mg/ml BSA and 2% glycerol. The increase in TAMRA and Fluorescein fluorescence, i.e. SD and polymerase activity, respectively, has been measured as relative fluorescence units over time and is depicted as thousandth (milli) relative fluorescence unit per minute (mRFU/min). For SD 63 ng of each protein and for polymerase activity 36 ng of each protein were used

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