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Table 1 Forward (forw) and reverse (rev) primer sequences for cloning of wild type enzymes (wt) and for site-directed mutagenesis (substitution of Asp by Ala at position 422, D422A) of DNA polymerase I large fragments

From: Characterization and engineering of a DNA polymerase reveals a single amino-acid substitution in the fingers subdomain to increase strand-displacement activity of A-family prokaryotic DNA polymerases

Primer

Sequence (5′ to 3′ direction)

Psychrobacillus sp.

 PB_wt_forw

CACCACAGAAGTAGCATTCGAGATTGTT

 PB_wt_rev

TTACTTCGTGTCATACCAAGATGAACC

Geoacillus stearothermophilus

 Gbst_wt_forw

ACCATCATGGATCCGGCGCCAAAATGGCATTTACCCT

 Gbst_wt_rev

CATCCGCCAAAACAGCCTTATTTGGCATCATACCAGG

 Gbst_D422A_forw

GTGTATGGCATCAGCGCTTATGGTCTGGCACAG

 Gbst_D422A_rev

CTGTGCCAGACCATAAGCGCTGATGCCATACAC

Ureibacillus thermosphaericus

 Ureibac_wt_forw

ACCATCATGGATCCGGCGCAGCACTGAGCTTTAAAAT

 Ureibac_wt_rev

CATCCGCCAAAACAGCCTTATTTGGCATCGTACCAGG

 Ureibac_D422A_forw

CTATGGCATCAGCGCTTATGGTCTGAGCC

 Ureibac_D422A_rev

GGCTCAGACCATAAGCGCTGATGCCATAG