Oligonucleotide | Sequence (5′ to 3′ direction) |
---|---|
Single-nucleotide incorporation assay | |
Template | ATTGAGTGGAGATAGTATCGTAGGGTAGTATTGGTGGATA |
Primer | [FAM] TATCCACCAATACTACCCT |
Polymerase activity assay | |
Template | GGCCCGTDabcylAGGAGGAAAGGACATCTTCTAGCATFlcACGGGCCGTCAAGTTCATGGCCAGTCAAGTCGTCAGAAATTTCGCACCAC |
Primer | GTGGTGCGAAATTTCTGAC |
Strand-displacement activity assay | |
Template | [BHQ2]ATTGAGTGGACAAAGTATCGTAGGGTAGTATTGGTGGATA |
Reporter strand | CGATACTTTGTCCACTCAAT [TAMRA] |
“Cold” primer | TATCCACCAATACTACCCT |