Fig. 3
p53 response in ROSC-treated hESCs. a Time course of p53 stabilization in hESCs upon ROSC (20 μM) treatment was analyzed by Western blotting with anti-p53 antibody. Actin served as loading control. Bar graph shows densitometric quantification (lower panel). b Immunofluorescence staining of untreated, ROSC-treated (20 μM) or Camptothecin-treated (1 μM) H9 cells. Figure shows representative images of hESCs stained with antibodies against γH2AX, p53 and phospho-p53Ser15 (p53pSer15). Nuclei were counterstained with DAPI. The scale bars represent 100 μm. c A time-course analyses of p53 targets mdm2, p21Cip1, puma and noxa mRNA expression levels by Real Time RT-PCR in ROSC-treated or untreated hESCs. Rpl7 expression was used as normalizer