Fig. 6

Autophagy flux inhibited by the combinations of PTX with sub-lethal doses of EMSO. a Representative Western blot showing the relative levels of p62 protein (N = 3). Densitometric analysis of the levels of p62 protein is shown in the bottom in A. Gray column stands for A2780 cells, black column represents A2780/T cells (*P < 0.05). b The mRNA level of ATG5 was determined using a real-time PCR kit. Data represent the mean ± SD from three independent experiments (*P < 0.05). c Autophagy evaluated by GFP-LC3B puncta formation in two EOC cells transfected with an Ad- mCherry-GFP-LC3B construct for 48 h. d Quantification of GFP-LC3B puncta showing means + SE using Image Pro software (Version premier). A representative experiment is shown and quantification is based on three repeats (**p < 0.01 vs control). IC50 values of paclitaxel, EMSO plus paclitaxel, and EMSO as well as combination index values of synergistic activity in A2780 and A2780/T cell lines