Fig. 5

miR-185-5p inhibition rescues the inhibition of matrix mineralization in osteoblasts by H19 knockdown. a The mRNA and protein expression of OCN, ALP and Collagen I in H19 knocked-down MC3T3-E1 cells transfected with miR-185-5p inhibitor and NC by qPCR and western blot. GAPDH was used for normalization. b ELISA analysis of the amount of Collagen I and BALP protein in the supernatant of in H19 knocked-down MC3T3-E1 cells transfected with miR-185-5p inhibitor and NC. c Representative images of ALP staining of transfected MC3T3-E1 cells. Scale bars, 800 μm. d Staining of calcium deposition by Alizarin Red in MC3T3-E1 cells transfected with si-H19, miR-185-5p inhibitor and NC. Scale bars, 800 μm. The asterisks show difference significant as ** p < 0.01, * p < 0.05