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Table 2 List of the strains prepared and used in this study

From: Construction of strains to identify novel factors for regulation of centromeric cohesion protection (CCP) and sister kinetochore mono-orientation (SKM)

Strain ID

Nick Name

Genotype

Background

SGY229

WT CENV-GFP

Mat-a, leu2::tetR-GFP::LEU2::TetO-HIS3

CRY1

SGY4104

CRY1-pRS406-control

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL -URA

CRY1

SGY4105

CRY1-pRS406-GAL-CDC5

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL -CDC5-URA

CRY1

SGY4106

CRY1-pRS406-GAL-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - MAM1-URA

CRY1

SGY4107

CRY1-pRS406-GAL-CDC4-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - CDC4-MAM1-URA

CRY1

SGY4108

CRY1-pRS406-GAL-REC8

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - REC8-URA

CRY1

SGY4109

CRY1-pRS406-GAL-SPO13

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - SPO13-URA

CRY1

SGY4110

CRY1-pRS406-GAL-REC8-SPO13

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL - REC8-SPO13-URA

CRY1

SGY4111

CRY1-pRS405

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU

CRY1

SGY4112

CRY1-pRS405-GAL-SPO13

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-SPO13-LEU

CRY1

SGY4113

CRY1-pRS405-GAL-CDC5

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU

CRY1

SGY4114

CRY1-pRS405-GAL-CDC5-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU

CRY1

SGY4119

CRY1-pRS405-GAL-SPO13-REC8

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-SPO13-REC8-LEU

CRY1

SGY4129

Cdc16–123-pRS405

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU

Cdc16–1

SGY4130

Cdc16–123-pRS405-CDC5

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU

Cdc16–1

SGY4131

Cdc16–123-pRS405-CDC5-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU

Cdc16–1

SGY4132

Cdc16–1-pRS405

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU

Cdc16–123

SGY4133

Cdc16–1-pRS405-CDC5

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU

Cdc16–123

SGY4134

Cdc16–1-pRS405-CDC5-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU

Cdc16–123

SGY4135

Cdc20–1-pRS405

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-LEU

Cdc20–1

SGY4136

Cdc20–1-pRS405-CDC5

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-LEU

Cdc20–1

SGY4137

Cdc20–1-pRS405-CDC5-MAM1

Mat-a ade2–1 ura3–1 leu2–3112,trp1,his3–11::GAL-CDC5-MAM1-LEU

Cdc20–1