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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Design and structural characterisation of olfactomedin-1 variants as tools for functional studies

Fig. 5

Comparison of Olfm1Olf domain in the Ca2+- and Na+-bound state in grey with the previously-published apo state in teal [6]. The switch loop (violet) is only resolved in the Ca2+- and Na+-bound state. In the apo state, the negatively-charged side chains of Asp356, Glu404 and Asp453 are pushed outward in the absence of compensating positive charges from the Na+ and Ca2+ ions (indicated by arrows in the right panel). This most likely destabilises the conformation of the switch loop (violet) via Tyr347, which consequently is unstructured (indicated by a dashed line in the left panel) and thus not observed in the electron density of the apo form [6]. The intra-chain disulfide between Cys227 and Cys409 is shown in stick representation

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