Fig. 6

Characterisation of CB-MSC adipogenic differentiation under normoglycaemic and hyperglycaemic conditions. a Lipid vacuole formation, as visualised by LipidTOX™ Green Neutral Lipid Stain, by CB-MSCs cultured for 21 days in adipogenic and basal (control) medium. At PD15 and PD50, CB-MSCs demonstrated prominent LipidTOX™ staining under both normoglycaemic and hyperglycaemic conditions. CB-MSCs at PD100 and PD200 exhibited fewer lipid vacuoles and a reduced capacity to differentiate into adipocytes. Assessment of late adipogenic marker, b adiponectin and c PPARγ, expression by CB-MSCs under normoglycaemic and hyperglycaemic conditions, by Q-PCR. Although baseline (day 0) analyses demonstrated no differences in adiponectin or PPARγ expression between normal and high glucose conditions, both showed significantly increased expression by CB-MSCs at PD15 and PD50 under normoglycaemic conditions at day 21. Significantly reduced adiponectin and PPARγ expression were identified at PD100 and PD200, under both normal and high glucose conditions. Significance between CB-MSC populations are shown, ***p < 0.001, *p < 0.05. Scale bar = 100 μm