Table 1 Primers sequences for RT-PCR and Q-PCR analysis
Gene | Primer Sequence | Length (bp) | Application |
|---|---|---|---|
p53 | F: ACAGCGTGGTGGTACCGTAT R: GGAGCTGTTGCACATGTACT | 83 | Q-PCR |
p21waf1 | F: TCTTGCACTCTGGTGTCTCA R: GGGCTTTCTCTTGCAGAAG | 147 | Q-PCR |
p16INK4A | F: TGCAGATAGACTAGCCAGGGC R: CTCGCAGTTCGAATCTGCAC | 184 | Q-PCR |
GAPDH | F: GCAAGAGAGAGGCCCTCAG R: TGTGAGGGAGATGCTCAGTG | 74 | Q-PCR |
rTERT | F: GCTCCGGTTACACAGCAGCCCCTGGCA R: GGTCCAGAGCACGCACACGCAGCACGA | 752 | RT-PCR |
TR | F: CTCCGCCCGCTGTTTTTCTCGCTGACT R: GCCACCGAACTCAGGGACCAGTTCCGT | 300 | RT-PCR |
CD73 | F: TCCCGCGGCTGCTACGGCACCCAAGTG R: ACCTTGGTGAAGAGCCGGGCCACGCCG | 204 | RT-PCR |
CD90 | F: CCTGACCCGAGAGAAGAA R: TGAAGTTGGCTAGAGTAAGGA | 125 | RT-PCR |
CD105 | F: CGGTCTCCAGCTGCGGTGGTGGGCTCC R: CACTGCCACCACGGGCTCCCGCTTGCT | 896 | RT-PCR |
CD34 | F: GTCACACTGCCTACTACTTC R:TCCTCGGATTCCTGAACAT | 210 | RT-PCR |
CD45 | F: AGCAATACCAGTTCCTCTATGA R: TCCGTCCACTTCGTTATGA | 113 | RT-PCR |
CD106 | F: TCCACACTGACGCTGAGCCCTGTGGGTG R: CTCCGGCATCCTGCAGCTGTGCCTTGCG | 898 | RT-PCR |
CD146 | F: GCAGCGCCACGGGTGTGCCAGGAGAGG R: CCCCACTGTGGTGCTTCTGGGCGGGCT | 900 | RT-PCR |
Nanog | F: GGGGATTCCTCGCCGATGCCTGCCGTT R: GGGATACTCCACCGGCGCTGAGCCCTT | 477 | RT-PCR |
Oct4 | F: GCCCACCTTCCCCATGGCTGGACACCT R: GCAGGGCCTCGAAGCGGCAGATGGTTG | 563 | RT-PCR |
Slug | F: CACTCCCCTCTGCCCAGCGGCCTTTCT R: GGCATGGGGGTCTGAAAGCTTGGGCTG | 171 | RT-PCR |
Snail | F: GCGAGCTGCAGGACGCGTGTGTGGAGT R: CGGCAAAGGCACGGTTGCAGTGGGAGC | 597 | RT-PCR |
β-actin | F: TGAAGATCAAGATCATTGCTCCTCC R: CTAGAAGCATTTGCGGTGGACGATG | 155 | RT-PCR |
Osterix | F: GCTTTTCTGTGGCAAGAGGTTC R: CTGATGTTTGCTCAAGTGGTCG | 136 | Q-PCR |
OCN | F: AAGCCCAGCGACTCTGAGTCT R: CCGGAGTCTATTCACCACCTTACT | 75 | Q-PCR |
Adiponectin | F: GAATCATTATGACGGCAGCAC R: CTTGGAGCCAGACTTGGTCTC | 224 | Q-PCR |
PPARγ | F: GGAAGCCCTTTGGTGACTTTATGG R: GCAGCAGGTTGTCTTGGATGTC | 174 | Q-PCR |
β-actin | F: TGAAGATCAAGATCATTGCTCCTCC R:CTAGAAGCATTTGCGGTGGACGATG | 108 | Q-PCR |