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Fig. 1 | BMC Molecular and Cell Biology

Fig. 1

From: Binding of Brucella protein, Bp26, to select extracellular matrix molecules

Fig. 1

Binding of Bp26 protein to immobilized ECM components. Depiction of binding of collagen type I, fibronectin, vitronectin, laminin and bovine serum albumin (negative control) immobilized on a microtitre ELISA plate after overnight incubation at 4 °C with Bp26 protein (5 μg/ μl). Different concentrations of the molecules were detected by peroxidase reaction using anti-Bp26 mouse serum (diluted 1:1000) and rabbit anti-mouse IgG peroxidase conjugate and peroxidase substrate. The measures represent the average of each independent test after subtraction of the background value obtained in the absence of each the ECM molecules. Bars indicate standard errors presented as the means ± SD for each of the four tests

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