Fig. 3
From: Molecular effects of dADD1 misexpression in chromatin organization and transcription
Overexpression of dADD1 proteins changes HP1a distribution in whole salivary glands. a Salivary gland immunostaining with HP1a antibody from wild-type (wt), UAS-dADD1; Sgs3-GAL4 (>dAdd1), Sgs3-GAL4/UAS-dADD1a (>dAdd1a) and Sgs3-GAL4/UAS-dADD1b (>dADD1b), HP1a (red signal), DNA (grey signal) and merge scale bar 100 μm. The right column shows a magnified image of a single nucleus with scale bar 5 μm b Percentage of nuclei with three different distributions of HP1a signal, “n” represents the number of nuclei analyzed in each genotype. Kruskal-Wallis Test was performed to determine the significance, p < 0.0001****, indicating a scale “1” for focal, “2” for two foci and “4” for wide. c Classification of HP1a (red signal) distributions found in the nuclei, scale bar 5 μm