Fig. 4From: Robust high-throughput assays to assess discrete steps in ubiquitination and related cascadesFlowchart of optimized conditions for each Alpha assay. Stepwise procedures are shown for: a PCNA ubiquitination, b Uba1~ubiquitin thioester formation, c Rad6~ubiquitin thioester formation, d Rad18 autoubiquitination. Detailed conditions are described in MethodsBack to article page