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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Basic biochemical characterization of cytosolic enzymes in thymidine nucleotide synthesis in adult rat tissues: implications for tissue specific mitochondrial DNA depletion and deoxynucleoside-based therapy for TK2-deficiency

Fig. 6

Thymidylate synthase activity (a) and protein level (b) in rat tissues. Equal volume of cytosolic and mitochondrial extracts was used in the TS activity (expressed as pmol/min/mg) and radiolabelling of TS protein experiments using [32P]-5-fluoro-dUMP. The intensity of each band was quantified and normalized to the amount of protein used in the experiments and expressed as arbitrary unit/mg protein. A representative autoradiograph image is shown. Results are mean ± SD. Number 1 to 7 represents liver, heart, brain, kidney, spleen, lung and skeletal muscle

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