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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: The mitochondrial outer membrane protein SYNJ2BP interacts with the cell adhesion molecule TMIGD1 and can recruit it to mitochondria

Fig. 6

Ectopic Myc-SYNJ2BP recruits TMIGD1 to mitochondria in a PDZ domain-dependent manner. MDCKII-TetOFF cells stably expressing either Flag-TMIGD1 WT (a), Flag-ΔD1-TMIGD1 (b), or Flag-ΔD1-TMIGD1/Δ5 (c) were transiently transfected with Myc-SYNJ2BP. Cells were stained with antibodies against Myc-SYNJ2BP (anti Myc, SantaCruz #sc-789G), Flag-TMIGD1 (anti Flag, Sigma #F7425), and either endogenous β-catenin to visualize cell-cell contacts or with mitotracker to visualize mitochondria, as indicated. The bar graphs in each panel show quantifications of Flag-TMIGD1 localization in Myc-SYNJ2BP-expressing cells (TMIGD1 co-localization with Myc-SYNJ2BP in mitochondria vs TMIGD1 localization in the cytoplasm (panel a), or TMIGD1 co-localization with Myc-SYNJ2BP in mitochondria vs TMIGD1 localization at β-catenin-positive cell contacts (ΔD1-TMIGD1 and ΔD1-TMIGD1/Δ5, panels b and c). Data are indicated as the fraction of Myc-SYNJ2BP-positive cells that are positive for TMIGD1 at mitochondria vs cytoplasm (a) or cell contacts (b, c). Data were obtained from at least three independent experiments with at least 50 cells analyzed. Error bars indicate standard deviations between experiments. Scale bars: 10 μm

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BMC Molecular and Cell Biology

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