Fig. 3

Violet light strongly impairs the growth of B. subtilis cells. Time lapse microscopy of exponentially growing B. subtilis cells. a) Cells were grown on the agarose pads for 130 min (after having reached mid-exponential phase in liquid culture) and were then subjected to 3% 405 nm laser light for 15 s (PALM illumination), as indicated by the 4 central colored images. They did not continue growth for at least 60 min after laser illumination. b) Cells were grown for 30 min and subjected to 15 s of 0.1% laser light (lower end of PALM illumination, indicated by the 5 black images), after which they continued to grow. The sizes of the scale bars are indicated in the images