Fig. 4From: Bacterial cell growth is arrested by violet and blue, but not yellow light excitation during fluorescence microscopyEscherichia coli cells are sensitive to CFP but not to YFP time lapse imaging. Time lapse microscopy of exponentially growing E. coli cells. “pre” refers to bright field image taken before the 15 min time lapse series, in which cells were exposed to 1000 ms of a) bright field excitation, b) YFP and c) CFP excitation at 1 min intervals. Note that cells were placed on medium-containing agarose pads. Therefore, changes in cell morphology observed after CFP excitation did not result from the desiccation of cells. White bars: 2 μmBack to article page