Fig. 3
From: Interaction between NSMCE4A and GPS1 links the SMC5/6 complex to the COP9 signalosome
Colocalization between GPS1 and SMC5/6 components. (A-C) Co-localization of HA-tagged NSMCE4A and FLAG-tagged GPS1 in HeLa cells. (a) NSMCE4A localizes predominantly in the nucleus. NSMCE4A and GPS1 colocalize in the nucleus and in some cases in the cytoplasm as well, indicating, that NSMCE4A tends to localize in the cytoplasm in the presence of GPS1. (b) Nucleocytoplasmic distribution of NSMCE4A in the absence and presence of FLAG-tagged GPS1. NSMCE4A tends to be more nuclear if GPS1 is not overexpressed. Error bars indicate the standard error of mean (SEM). The p-value of t-test is indicated. The p-value of non-parametric Mann-Whitney test is 5.41 × 10− 6. (c) Relationship between the nucleocytoplasmic ratio of GPS1 and that of NSMCE4A. The plot indicates that the lower nucleocytoplasmic ratio of GPS1 corresponds to lower nucleocytoplasmic ratio of NSMCE4A. Pearson correlation coefficient: 0.639, Spearman correlation coefficient: 0.655. Colocalization of (d) NSMCE4A and (e) SMC6 with GPS1 during interphase and metaphase using antibodies raised against the endogenously expressed proteins. Size bars = 10 μm. See Supplemental Figure S1 for complementary data