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Fig. 4 | BMC Molecular and Cell Biology

Fig. 4

From: Klotho is regulated by transcription factor Sp1 in renal tubular epithelial cells

Fig. 4

Sp1 recovers morphological and the expression of E-cadherin, α-SMA, Fibronectin of TGF-β1-induced HK-2 cells. HK-2 cells (a-b) were treated with increasing amount of TGF-β1. (a-b) 48 h after treatment, total proteins were isolated and the protein of α-SMA, E-cadherin, Fibronectin, Sp1 and Klotho were detected by Western blots. (c) HK-2 cells were treated with TGF-β1(10 ng/mL) for 0.5, 4, 8 h, and the protein of Sp1 was detected by Western blots. (d-e) HK-2 cells were transfected with pcDNA3-Sp1 plasmid followed by treating with TGF-β1 for 48 h, effects of pcDNA3-Sp1 on morphology and α-SMA expression of HK-2 cells. (f) pcDNA3-Sp1 plasmid or empty vector were transfected into HK-2 cells followed by treating with TGF-β1. 48 h after treatment, total proteins were isolated and the protein levels of Klotho, E-cadherin, α-SMA, and Fibronectin were detected by Western blots. Data are means ± SD, ***p < 0.001, **p < 0.01. NS, statistically nonsignificant

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