Fig. 3
From: Methotrexate impaired in-vivo matured mouse oocyte quality and the possible mechanisms
Effects of MTX on the meiotic spindles and transcripts for MAD2 and Sgo1. Oocytes were labelled with antibodies against β-tubulin (green) and were counterstained with Hoechst 33342 (blue). Different spindle morphologies are shown in a-d. The rates of abnormal spindle are shown in e. mRNA transcript levels of MAD2 and Sgo1 are shown in f. The oocyte numbers for analyzing the rates of abnormal spindle were 384 in the control group, and 275 oocytes in the MTX group. * p<0.05