Fig. 4

The inverse relation between HOTAIR and WIF-1 expression is demonstrated by WIF-1 mRNA and protein expression in SW1353 cells at 48 h after (a) siRNA-mediated knockdown of HOTAIR or (b) over-expression of HOTAIR by retrovirus infection, as well as by (c) a dual luciferase reporter assay at 36 h after transfecting HOTAIR over-expressing or control SW1353 cells with a vector containing the WIF-1 promoter. The HOTAIR knockdown and over-expression groups are expressed as a fold difference from their respective controls. (d) Schematic illustrating primer locations in the WIF-1 promoter. (e) ChIP assay showing enrichment of H3K27 at the WIF-1 promoter. The PCR products were analysed by gel electrophoresis. *P < 0.05