Fig. 7

Chromatin immunoprecipitation (ChIP) analysis. ChIP places Pol II on rDNA 18S and 25S sequences during stationary growth phase and on BMH21 stimulation. (a-d) Chromatin immunoprecipitation with polymerase II and TFIIB specific antibodies. Cells were cross-linked, and chromatin was sheared by sonification. RNA Polymerase II mAb CTD4H8 (Epigentek) was used to precipitate DNA-protein complex. qPCR was performed using two different sets of specific primers, 18S and 25S. A control (non-immune) IgG antibody was used as negative control. BMH21- indicates mid-log cells not exposed to BMH21. Error bars represent standard deviation from three different experiments. P values were generated by Student’s test, **p < 0.01, ***p < .0002, ****p < .0001. e Amplicons location on rDNA gene. See Supplementary Table 1 for primer information