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Table 1 Mutagenesis of Pxkmo and Pxcardinal using CRISPR/Cas9 system

From: Ommochrome pathway genes kynurenine 3-hydroxylase and cardinal participate in eye pigmentation in Plutella xylostella

 

G0

G1

Injected eggs

Surviving pupae (%)

Visible mosaic adults (%)

Total number of G0s crossed (pools)

G0 pools showing mutant G1

Minimal germline transformation efficiency

Pxkmo

222

94 (42.3)

67 (71.3)

94 (8)

8

17.0% (16/94)

Pxcardinal

182

55 (30.2)

31 (56.4)

28 (8)

8

57.1% (16/28)