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Fig. 8 | BMC Molecular and Cell Biology

Fig. 8

From: Selecting suitable reference genes for qPCR normalization: a comprehensive analysis in MCF-7 breast cancer cell line

Fig. 8

Summary of Normalization of GOI 1, GOI 2, AURKA and KRT19 by different recommended reference genes pairs. Green circles indicate successful normalization of the gene of interest by respective reference gene pair (a) Normalization of the four genes of interest by selected reference genes in pairs of 2 in culture A1 (b) Normalization of the four genes of interest by selected reference genes in pairs of 2 in culture A2 (c) Normalization of the four genes of interest by selected reference genes in pairs of 3 in culture A1. (d) Normalization of the four genes of interest by selected reference genes in pairs of 3 in culture A2. Blue box indicates normalization by GAPDH-CCSER2 pair (recommended pair in culture A1) while Pink box indicates normalization by GAPDH-RNA28S pair (recommended pair in culture A2). Red box indicates the best reference gene triplet (GAPDH-CCSER2-PCBP1)

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