Fig. 5

WRN depletion in HeLa cells does not results in altered levels of the major mRNA export receptors. a Extracts prepared from shCTR and shWRN HeLa cells 72 h after dox induction were analyzed by Western blot using the indicated antibodies. Molecular size markers (in KiloDaltons) are shown. (Lower panel) Bands intensities in four biological replicates were quantitated using ImageJ (NIH) and plotted into the graph. Two-way ANOVA followed by Sidak’s multiple comparisons test was used to calculate the significance of the means and normalized against tubulin using GraphPad Prism. The error bars represent the mean ± SEM (n = 4).; ns, no significant difference. b Western blot showing the TREX-1 subunit THOC1, UAP56 and eIF4E in WRN-depleted and control HeLa cells. (Lower panel) semiquantitative analysis of protein levels as described in (a). c Western blot showing the TREX-1 component THOC2, ALYREF and CBP80 in WRN-depleted and control HeLa cells. (Lower panel) semiquantitative analysis of protein levels as described in (a). d Western blot analysis of the mRNA export receptor GANP in WRN-depleted and control HeLa cells. Right panel shows semiquantitative analysis of protein levels as described in (a). In the plots of Figs. (b), (c) and (d) Two-way ANOVA followed by Sidak’s multiple comparisons test were used to calculate the significance of the means and normalized against tubulin using GraphPad Prism. The error bars represent the mean ± SEM (n = 3). ns, no significant difference. Molecular size markers (in KiloDaltons) are shown