Fig. 5

The synthetic lethal assay used to identify novel mutant yeast whose survival was dependent on the ECM14 gene. a (1) Wild-type ECM14 was first moved from the yeast genome into the pSLS1 vector (pSLS1-ECM14). (2) Random genomic point mutations were introduced throughout the yeast genome using ethyl methansulfonate (EMS). (3) Colonies dependent on ECM14 were identified as non-sectored red colonies. b Putative synthetic lethal mutants that initially produced the desired non-sectored red phenotype were re-streaked for single colonies on galactose media to evaluate the development and maintenance of the red phenotype. Asterisks indicate colonies that were screened on media containing Ura + CFW