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Fig. 6 | BMC Molecular and Cell Biology

Fig. 6

From: Biochemical and genetic analysis of Ecm14, a conserved fungal pseudopeptidase

Fig. 6

The functional rescue assay workflow. (1) A diploid yeast strain is produced by crossing a wild-type haploid [pRS316-NatMX] strain with a haploid synthetically lethal strain (gene-y, ecm14::KanMX). Diploids are selected for by growth on YPD/G418/NTC. (2) Growth on SC + 5FOA allows for selection against the now unnecessary pRS316-NatMX and pSLS1-ECM14 plasmids. (3) Cells are transformed with the pSLS1 or pSLS1-ECM14 plasmids followed by (4) random sporulation and germination of these haploid spores on YPGal

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