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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein

Fig. 3

The functional verification of Haplotypes AA and BB at the individual level in pigs. a Functional analysis of differentially expressed genes between genotypes AA and BB in pigs (n = 10 for AA; n = 16 for BB). b Q-PCR validation of the differentially expressed genes (n = 6 for AA; n = 6 for BB). β-actin was used as the internal control for Q-PCR. Triplicate samples were analyzed for each treatment, and the results are presented as the mean ± s.e.m. **, P < 0.01. c The protein interaction network of DEGs. The red nodes represented genes that were up-regulated in individuals with genotype BB, and the green nodes represented genes that were down-regulated. The white nodes represented genes that were not significantly different between genotypes AA and BB. d Western blotting detection of the protein levels of CD4 and p-p65 of the two haplotypes (genotype AA, n = 5; genotype BB, n = 5) using anti-CD4 and anti-p65. β-actin was used as internal control

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BMC Molecular and Cell Biology

ISSN: 2661-8850

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