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Fig. 5 | BMC Molecular and Cell Biology

Fig. 5

From: Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 protein

Fig. 5

The identification of the key mutation sites that led to the functional difference between haplotypes A and B in pigs. a Model of mutant vectors of the CD4 gene. Mut 1, Mut 2, Mut 3, Mut 4, and Mut 5 indicated that 2, 5, 7, 9, and 11 SNPs mutated from haplotype A to haplotype B, respectively. Mut 6 and Mut 7 indicated that 2 and 5 SNPs mutated from haplotype B to haplotype A, respectively. Red and blue squares indicate the amino acids that corresponded with the SNPs. All mutants were inserted into a pEGFP-n1 vector for the construction of the GFP fusion protein. b Confocal images of the immune stain of GFP (green) protein. Nucleus was stained with DAPI (blue). Scale bars: 10 μm. c Western blotting results of the CD4-GFP fusion protein at 24 h and 48 h post-transfection using GFP antibody. β-actin was used as internal control

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