Fig. 5From: Three functional mutation sites affect the immune response of pigs through altering the expression pattern and IgV domain of the CD4 proteinThe identification of the key mutation sites that led to the functional difference between haplotypes A and B in pigs. a Model of mutant vectors of the CD4 gene. Mut 1, Mut 2, Mut 3, Mut 4, and Mut 5 indicated that 2, 5, 7, 9, and 11 SNPs mutated from haplotype A to haplotype B, respectively. Mut 6 and Mut 7 indicated that 2 and 5 SNPs mutated from haplotype B to haplotype A, respectively. Red and blue squares indicate the amino acids that corresponded with the SNPs. All mutants were inserted into a pEGFP-n1 vector for the construction of the GFP fusion protein. b Confocal images of the immune stain of GFP (green) protein. Nucleus was stained with DAPI (blue). Scale bars: 10 μm. c Western blotting results of the CD4-GFP fusion protein at 24 h and 48 h post-transfection using GFP antibody. β-actin was used as internal controlBack to article page