Fig. 3

The effect of E343 mutation on MAPK signaling. The stably overexpressing HeLa cells were serum-starved and then exposed to SDF-1 (50 nM) and over a range of time periods (5 min to 60 min). Western blotting was performed for phosphorylated ERK1/2 (a), p38 (c) and JNK1/2/3 (e). Total ERK1/2, p38 and JNK were used as loading control. The relative phosphorylation level was quantified using ImageJ software and showed in (b) for ERK1/2, (d) for p38 and (f) for JNK1/2/3, respectively. Bars represent the mean value ± standard deviation of at least three independent experiments. * P < 0.05; ** P < 0.01; *** P < 0.001