Table 1 Primers used in RT-qPCR
Gene (human) | Forward and reverse primers (5′-NNN-3′) |
|---|---|
GAPDH | F: 5′-TCGACAGTCAGCCGCATCTTCTTT-3′ R: 5′-ACCAAATCCGTTGACTCCGACCTT-3’ |
Glypican-1 | F: 5’-TATTGCCGAAATGTGCTCAAGGGC-3′ R: 5′-ATGACACTCTCCACACCCGATGTA-3’ |
Glypican-2 | F: 5’-TCCTTTCTGGTTCACACACTGGCT-3′ R: 5′-ACAGGCCATTGAATATGAGGGCGT-3’ |
Glypican-3 | F: 5’-TGAAAGTGGAGACTGCGGTGATGA-3′ R: 5′-TCCCGAGGTTGTGAAAGGTGCTTA-3’ |
Glypican-4 | F: 5’-TCGGAGATGTCCCTCGCAAATTGA-3′ R: 5′-TCTTCAACAGGGCATGGGTACACT-3’ |
Glypican-5 | F: 5’-ACTGGCATGCATATATCCGGTCGT-3′ R: 5′-TGAGGTGAGCCTGTAACACAGCAT-3’ |
Glypican-6 | F: 5’-CTTCATTGCTGCCAGGACCTTTGT-3′ R: 5′-AGCCCTTCATGACGTTGAGACAGT-3’ |
β-catenin | F: 5’-CTTCACCTGACAGATCCAAGTC-3′ R: 5′-CCTTCCATCCCTTCCTGTTTAG-3’ |