Fig. 3
Flow cytometry to identify the epithelial cells, and mRNA analysis to verify the fibroblasts’ identity. a-f example dot plots. Flow cytometry scatter plots of the adherent epithelial cells side scatter (SSC)/ Fluorescence dot plots of each marker; epithelial cells were found positive for CD29 (a) and CD44 (b) lineages markers and also for CD90 (c), CD34 (d), CD326 (e), and CD133 (f) stemness markers; g) mRNA expression of genes involved in fibroblast characterization was determined by qPCR. Transcript levels were normalized to the ACTB reference gene using log2 (2-ΔCt) method. The data are presented as mean ± standard deviation (SD). The graph bar shows expression level of the genes CD90, CD73, CD105, CD45, and CD34 of cultured fibroblasts. Gene expression was confirmed by 1.5% agarose gels