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Fig. 3 | BMC Molecular and Cell Biology

Fig. 3

From: sFLT01 modulates invasion and metastasis in prostate cancer DU145 cells by inhibition of VEGF/GRP78/MMP2&9 axis

Fig. 3

Analysis of sFLT01 effect on tube formation ability in HUVECs. Tube formation assay was performed on the human umbilical vein endothelial cells (HUVECs). Cells were seeded into a 96-well plate, pre-coated with Matrigel, and treated with conditioned media containing sFLT01 CM and control CM for 18 h. a Representative phase contrast picture of tubular network of HUVEC cells in control group. b Corresponding skeletons of tubular networks identifying segments (yellow color), mesh (blue color) and nodes (red color) by AngioTool plugin (ImageJ software) in control group. c Representative phase contrast picture of tubular network of HUVEC cells in sFLT01 conditioned media group. d Corresponding skeletons of tubular networks in sFLT01 group. e Quantification of the number of master junctions and (f) meshes using imageJ software. Four randomly picked field from different wells were evaluated for the number of junctions and closed meshes (n = 4*P < 0.05)

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