Fig. 5

Temperature × pool interactions for MYOD (A), MYOG (B), IGF1 (C) and AREG (D) mRNA expression analyzed in proliferating myoblasts of pool 5 and pool 20 after 72 h of permanent cultivation at 35°, 37°, 39° or 41 °C. Data (least square means and standard errors) are expressed as arbitrary units after normalization to RN18S expression as an endogenous reference gene. Significant differences within each pool are indicated by asterisks (*P < 0.05, **P < 0.01 or ***P < 0.001), and significant differences between the pools at the same temperature are explained below