Parameter | Temperature (T) | Pool (P) | T P | P P | T × P P | ||||
---|---|---|---|---|---|---|---|---|---|
35 °C | 37 °C | 39 °C | 41 °C | 5 | 20 | ||||
DNA content (μg/well) | 0.47 ± 0.04 a | 0.28 ± 0.04 b | 0.54 ± 0.04 a | 0.43 ± 0.04 ab | 0.44 ± 0.03 | 0.42 ± 0.03 | 0.006 | 0.606 | 0.930 |
Protein content (μg/well) | 20.62 ± 3.34 | 22.57 ± 3.34 | 21.35 ± 3.34 | 24.04 ± 3.34 | 21.68 ± 2.36 | 22.61 ± 2.36 | 0.894 | 0.785 | 0.869 |
DNA synthesis (Abs 450 nm) | 1.62 ± 0.07 a | 1.27 ± 0.07 b | 1.34 ± 0.07 ab | 1.11 ± 0.07 b | 1.31 ± 0.05 | 1.36 ± 0.05 | <  0.001 | 0.431 | 0.794 |
LDH activity (IU/L) | 41.85 ± 2.27 a | 24.92 ± 2.27 b | 22.39 ± 2.27 b | 26.85 ± 2.27 b | 26.30 ± 1.61 | 31.70 ± 1.61 | <  0.001 | 0.032 | 0.715 |
TUNEL+ cells (%) | 0.05 ± 0.01 | 0.02 ± 0.01 | 0.04 ± 0.01 | 0.07 ± 0.01 | 0.04 ± 0.01 | 0.04 ± 0.01 | 0.141 | 0.681 | 0.712 |