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Table 2 Biochemical properties (least square means ± standard errors) of growth after 72 h of proliferation

From: Effects of temperature on proliferation of myoblasts from donor piglets with different thermoregulatory maturities

Parameter

Temperature (T)

Pool (P)

T

P

P

P

T × P

P

35 °C

37 °C

39 °C

41 °C

5

20

DNA content (μg/well)

0.47 ± 0.04 a

0.28 ± 0.04 b

0.54 ± 0.04 a

0.43 ± 0.04 ab

0.44 ± 0.03

0.42 ± 0.03

0.006

0.606

0.930

Protein content (μg/well)

20.62 ± 3.34

22.57 ± 3.34

21.35 ± 3.34

24.04 ± 3.34

21.68 ± 2.36

22.61 ± 2.36

0.894

0.785

0.869

DNA synthesis

(Abs 450 nm)

1.62 ± 0.07 a

1.27 ± 0.07 b

1.34 ± 0.07 ab

1.11 ± 0.07 b

1.31 ± 0.05

1.36 ± 0.05

<  0.001

0.431

0.794

LDH activity (IU/L)

41.85 ± 2.27 a

24.92 ± 2.27 b

22.39 ± 2.27 b

26.85 ± 2.27 b

26.30 ± 1.61

31.70 ± 1.61

<  0.001

0.032

0.715

TUNEL+

cells (%)

0.05 ± 0.01

0.02 ± 0.01

0.04 ± 0.01

0.07 ± 0.01

0.04 ± 0.01

0.04 ± 0.01

0.141

0.681

0.712

  1. Abs absorbance, LDH lactate dehydrogenase
  2. a, b Labeled least square means within a row with different letters differ (P < 0.05)