Gene | Temperature (T) | Pool (P) | T P | P P | T × P P | ||||
---|---|---|---|---|---|---|---|---|---|
35 °C | 37 °C | 39 °C | 41 °C | 5 | 20 | ||||
PCNA mRNA | 1.66 ± 0.05 a | 0.93 ± 0.05 b | 0.55 ± 0.05 c | 0.49 ± 0.05 c | 0.85 ± 0.04 | 0.96 ± 0.04 | <  0.001 | 0.060 | 0.174 |
DAD1 mRNA | 0.87 ± 0.05 | 0.78 ± 0.05 | 0.85 ± 0.05 | 0.96 ± 0.05 | 0.83 ± 0.04 | 0.89 ± 0.04 | 0.159 | 0.253 | 0.415 |
PPARGC1A mRNA | 1.40 ± 0.07 a | 0.91 ± 0.07 b | 0.39 ± 0.07 c | 0.39 ± 0.07 c | 0.97 ± 0.05 | 0.57 ± 0.05 | < 0.001 | < 0.001 | 0.105 |
SORBS1 mRNA | 0.66 ± 0.07 a | 0.91 ± 0.07 ab | 1.14 ± 0.07 b | 1.12 ± 0.07 b | 0.97 ± 0.05 | 0.94 ± 0.05 | <  0.001 | 0.667 | 0.503 |
Heat shock proteins | |||||||||
HSP25/27 mRNA | 0.81 ± 0.08 b | 0.80 ± 0.08 b | 0.79 ± 0.08 b | 1.50 ± 0.08 a | 0.94 ± 0.05 | 1.01 ± 0.05 | < 0.001 | 0.376 | 0.746 |
HSP70 mRNA | 0.57 ± 0.08 b | 0.46 ± 0.08 b | 0.75 ± 0.08 b | 1.26 ± 0.08 a | 0.83 ± 0.06 | 0.68 ± 0.06 | <  0.001 | 0.077 | 0.260 |
HSP90 mRNA | 0.87 ± 0.06 b | 0.76 ± 0.06 b | 0.86 ± 0.06 b | 1.29 ± 0.06 a | 0.90 ± 0.04 | 0.99 ± 0.04 | < 0.001 | 0.139 | 0.388 |
HSP70 protein | 0.18 ± 0.25 b | 0.15 ± 0.25 b | 0.24 ± 0.25 b | 2.33 ± 0.25 a | 0.70 ± 0.18 | 0.76 ± 0.18 | <  0.001 | 0.816 | 0.999 |
HSP90 protein | 2.23 ± 0.56 b | 2.59 ± 0.56 b | 3.96 ± 0.56 b | 7.09 ± 0.56 a | 3.97 ± 0.40 | 3.97 ± 0.40 | < 0.001 | 0.997 | 0.273 |
HSF1 protein | 5.33 ± 1.55 | 4.71 ± 1.55 | 1.63 ± 1.55 | 2.17 ± 1.55 | 3.32 ± 1.10 | 3.60 ± 1.10 | 0.282 | 0.855 | 0.820 |