Gene | Temperature (T) | Pool (P) | T P | P P | T × P P | ||||
---|---|---|---|---|---|---|---|---|---|
35 °C | 37 °C | 39 °C | 41 °C | 5 | 20 | ||||
Myogenic transcription factors | |||||||||
PAX7 | 0.92 ± 0.06 a | 0.76 ± 0.06 ab | 0.88 ± 0.06 ab | 0.66 ± 0.06 b | 0.91 ± 0.04 | 0.69 ± 0.04 | 0.022 | < 0.001 | 0.846 |
MYF5 | 0.96 ± 0.06 a | 0.71 ± 0.06 a | 0.45 ± 0.06 b | 0.38 ± 0.06 b | 0.78 ± 0.04 | 0.47 ± 0.04 | < 0.001 | < 0.001 | 0.426 |
MRF4 | 1.87 ± 0.19 | 1.87 ± 0.19 | 1.67 ± 0.21 | 0.88 ± 0.29 | 1.05 ± 0.15 | 2.09 ± 0.17 | 0.061 | < 0.001 | 0.291 |
Growth factors and growth factor receptors | |||||||||
MSTN | 0.85 ± 0.04 a | 0.69 ± 0.04 b | 0.42 ± 0.04 c | 0.37 ± 0.04 c | 0.70 ± 0.03 | 0.46 ± 0.03 | < 0.001 | < 0.001 | 0.233 |
IGF2 | 0.67 ± 0.07 b | 0.71 ± 0.07 b | 0.74 ± 0.07 ab | 1.02 ± 0.07 a | 1.01 ± 0.05 | 0.55 ± 0.05 | 0.015 | < 0.001 | 0.195 |
EGF | 0.79 ± 0.15 b | 0.71 ± 0.15 b | 1.09 ± 0.15 b | 2.42 ± 0.15 a | 1.27 ± 0.10 | 1.24 ± 0.10 | < 0.001 | 0.828 | 0.384 |
IGF1R | 0.89 ± 0.08 ab | 0.65 ± 0.08 b | 1.00 ± 0.08 a | 0.83 ± 0.08 ab | 0.71 ± 0.06 | 0.96 ± 0.06 | 0.043 | 0.006 | 0.082 |
EGFR | 1.22 ± 0.10 ab | 0.88 ± 0.10 b | 1.36 ± 0.10 a | 1.40 ± 0.10 a | 1.06 ± 0.07 | 1.37 ± 0.07 | 0.009 | 0.008 | 0.165 |